Pioneer Cemetery is one of the oldest cemeteries in Jefferson County and was located in the area of Avenues B & C South (now 2nd & 3rd Avenues South) and 32nd & 33rd Streets South. The cemetery was abandoned in the 1880s and later destroyed (and was used as city dump at one time). The cemetery was located on property owned by Benjamin P Worthington, who owned approximately 800 acres in what is now the Avondale/Lakeview area of Birmingham. There were approximately 100 graves in this cemetery,  Caucasian and African-American. Known burials in this cemetery are James Maynard Massey (1782-1857) and his wife's parents, Abraham (1743-1825) & Rebecca (1750-1845) Nations. Other Massey family members, Thomas Allen Massey and Martha Caroline Shackleford Massey, are also buried in this cemetery. Thomas McAdory (10 Sept 1776-8 July 1820) was originally buried in this cemetery but he was later reinterred to Cedar Hill Cemetery in Bessemer in approximately 1903. It is believed many early settlers of the Avondale area were buried in this cemetery. In an article from The Evening News, Birmingham AL, 18 June 1889, Pioneer Cemetery is described as "an old graveyard...long since abandoned." In an article from The Birmingham News, 17 November 1924, Pioneer Cemetery is described as "an ancient burying ground...overgrown with weeds...with neglected tombs." According to a cemetery survey undertaken by the University of Alabama, there were no burials there after about 1875. The cemetery was paved over and made into a miniature golf course by the about 1960, and was later turned into retail space and a parking lot in the mid-1980s. The cemetery, if it still existed, would be located in the parking lot of Mayer Lighting Showroom, and the back lot of the Birmingham Aids Outreach building (originally the Swann Chemical Building).

While some sources suggest that one of the other names for this cemetery is Roebuck-Mann Cemetery, that is a separate cemetery from Pioneer Cemetery. The Roebuck Mann Cemetery was located in Avondale and consisted of 3 graves. All 3 graves were moved to Oak Hill Cemetery in the 1920s.