Colonel Samuel Washington was the 3rd eldest child and second eldest Son of Augustine Washington, I & Mary (Ball) Washington, He Built "Harewood Plantation" (Jefferson County) Virginia now West Virginia, He was Col. Virginia Militia, JP and High Sheriff, born @ Wakefield, Westmoreland County, Virginia, he married 5 times:

1)Jane Champe ca. 1754 dau. of John Champe & Elizabeth Pope.
no Issue

2)Mildred Thornton ca. 1756 dau. of John Thornton & Mildred Gregory.
children:
Thornton Washington married twice 1st to Mildred Berry & 2ndly to his cousin Frances Townsend Washington
Tristram Washington died young

3)Louisa Chapman ca. 1762 dau. of Nathaniel Chapman & Constantia Pearson. no Issue

4)Anne Steptoe 24 Mar. 1764 dau. of James Steptoe & Hannah Ashton. & widow of Willoughby Allerton
children:
Ferdinand Washington died young
Frederick Augustus Washington died young
Lucinda Washington died young
George Steptoe Washington married Lucy Ann Payne
Lawrence Augustine Washington married Mary Dorcas Wood
Harriet Washington m. Col. Andrew Parks

5)Susannah Ferrin ca. 1778 dau. of John Ferrin and unknown Mother
children:
John Ferrin Washington died young